Brazilin Content, Antioxidative and Lipase Inhibition Effects of Sappanwood (Caesalpinia Sappan) from Indonesia

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Brazilin Content, Antioxidative and Lipase Inhibition Effects of Sappanwood (Caesalpinia Sappan) from Indonesia

Irmanida Batubara1,2, Mohamad Rafi1,2, Siti Sa'diah2,3, M. Agung Zaim2, Susi Indariani2, Tohru Mitsunaga4



1Departement of Chemistry, Faculty of Mathematics & Natural Sciences, Bogor Agricultural University, Indonesia.
2Biopharmaca Research Center, Institute of Research & Community Services, Bogor Agricultural University, Indonesia.
3Department Anatomy, Physiology, Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia
4Department of Applied Science, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

Issue Date : November 2010
Publisher  Journal of Chemistry and Chemical Engineering.
Series / Report No :
ISSN: 1934-7375
Physical Description :
2010, Vol. 4 No.10 p 50-55
Language : en



Determination of brazilin by High Performance Liquid Chromatography (HPLC) method and measurement of the antioxidative and lipase inhibition effects in the Caesalpinia sappan wood from various locations in Indonesia is described in this paper. Brazilin was separated from sample matrix using a reversed phase C18, Shim-pack VP column with the mobile phase in a gradient elution for 45 mm from 5% to 100% methanol in 0.05% aqueous trifluoroacetic acid. Brazilin in ethanolic extracts of C. sappan wood was in the range of 5.81 to 24.85 mg/g on dry-weight basis. Antioxidant and lipase inhibition activities expressed by IC50 values from all samples were in the range from 6.60 to 11.53 μg/mL and 50.76 to 203.21 μg/mL, respectively. Antioxidative and lipase inhibition potency of all ethanolic extracts were compared to vitamin C and chloramphenicol, isopropyl methyiphenol and tetracyclin, respectively.

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Mesh :


Caesalpinia sappan, sappanwood, brazilin, antioxidant activity, lipase inhibition activity, HPLC
How to cite this article


Batubara, I., M. Rafi, S. Sa'diah, M.A. Zaim, S. Indariani & T.Mitsunaga. 2010. Brazilin Content, Antioxidative and Lipase Inhibition Effects of Sappanwood (Caesalpinia Sappan) from Indonesia. Journal of Chemistry and Chemical Engineering, 4(10): 50-55.
url: http://biofarmaka.ipb.ac.id/publication/journal/144-brazilin-content-antioxidative-and-lipase-inhibition-effects-of-sappanwood-caesalpinia-sappan-from-indonesia



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