Biopharmaca BiofarmakaCharacterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16
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http://biofarmaka.ipb.ac.id/publication/journal/56-characterization-and-purification-a-specific-xylanase-showing-arabinofuranosidase-activity-from-streptomyces-spp-234p-16
Title
 
:
 

Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16

Authors
 
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Anja Meryandini1,, Trio Hendarwin1,♥♥, Fahrrurozi1, Alina Akhdiya2, Deden Saprudin3, and Yulin Lestari1,4
Corporate






 
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1Biology Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680.
2Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Bogor 16110.
3Chemistry Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680.
4Biopharmaca Research Center, Institute of Research and Community Services, Bogor Agricultural University. Kampus IPB Taman Kencana, Jl. Taman Kencana No. 3, Bogor 16128.

Issue Date : July 2009
Publisher : BIODIVERSITAS, Journal of Biological Diversity
Series / Report No :
 
ISSN: 1412-033X (printed edition) ISSN: 2085-4722 (electronic)

Physical Description :
 
2009 Vol. 09 No. 3, p 115-119
 
Language : en
Abstract




 
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Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90oC. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2½ hours at 90oC. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1,98 mg/mL and 523 μmol/minute/mg, respectively.

Source url

 
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http://biofarmaka.ipb.ac.id/publication/journal/56-characterization-and-purification-a-specific-xylanase-showing-arabinofuranosidase-activity-from-streptomyces-spp-234p-16

Mesh :

 

Keywords : xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces
How to cite this article



 
:





 
Meryandini, A., T. Hendarwin, Fahrrurozi, A. Akhdiya, D. Saprudin, and Y. Lestari. 2009. Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16. BIODIVERSITAS, Journal of Biological Diversity, 09(3): 115-119.
url: http://biofarmaka.ipb.ac.id/publication/journal/56-characterization-and-purification-a-specific-xylanase-showing-arabinofuranosidase-activity-from-streptomyces-spp-234p-16
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