Biopharmaca BiofarmakaTyrosinase Kinetic Inhibition of Active Compounds from Intsia palembanica
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JOURNAL 2012
Tyrosinase Kinetic Inhibition of Active Compounds from Intsia palembanica

Please use this identifier to cite or link to this item
http://biofarmaka.ipb.ac.id/publication/journal/631-tyrosinase-kinetic-inhibition-of-active-compounds-from-intsia-palembanica
Title  :

Tyrosinase Kinetic Inhibition of Active Compounds from Intsia palembanica

Authors

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L.K. Darusman1,2,*, I. Batubara1,2, T. Mitsunaga3, M. Rahminiwati1,4, E. Djauhari1,5 and K. Yamauchi3
Corporate









 
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1Biopharmaca Research Center, Bogor Agricultural University, Jl Taman Kencana No. 3 Bogor 16128, Indonesia
2Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, IPB Darmaga Campus, Bogor, Indonesia  
3Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan
4Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia
5Departement of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, IPB Darmaga Campus, Bogor, Indonesia
*Coressponding Author: L.K Darusman, Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No. 3, Bogor 16128. Telp +62-251-8373561 Fax +62+251+8347525 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Issue Date : December 5, 2012
Publisher : Research Journal of Medicinal Plant
Series / Report No :
ISSN 1819-3455 / DOI: 10.3923/rjmp.2012.615.620
 
Physical Description :
 
2012, Volume: 6, Issue: 8, Page No: 615-620
 
Language : en
Abstract















 
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Intsia palembanica is the most potential plant having tyrosinase inhibitor over 30 Indonesian plants that have been searched. It’s active compound namely (-)-robidanol, (+)-epirobidanol and 4’-dehydroxyrobidanol had been identified and showed the tyrosinase inhibitory activity. The study aims to obtain the kinetic enzyme inhibition type of the three active compounds. About 3 g of I. palembanica methanol extract was fractionated using n-hexane soluble part, ethyl-acetate soluble part and aqueous soluble part. Compounds were isolated for the most active part (ethyl-acetate soluble part) by silica gel column chromatography. Purification of fractions was performed using preparative High Performance Liquid Chromatography (HPLC). Kinetic inhibition against tyrosinase on monophenolase and diphenolase were analyzed by Lineweaver-Burk plot. The results showed that three active compounds showed different kinetic inhibition type on monophenolase and diphenolase. The kinetic characteristics of (-)-robidanol, the most active inhibitor, on monophenolase were Km increased and Vmax did not changed while on diphenolase were Vmax decreased and Km did not change. In conclusion, (-)-robidanol is a competitive inhibitor for monophenolase and a non-competitive inhibitor for diphenolase. The kinetic data is useful for further research on mechanism of action of the whitening agent that will be formulated.
Source url
 
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http://biofarmaka.ipb.ac.id/publication/journal/631-tyrosinase-kinetic-inhibition-of-active-compounds-from-intsia-palembanica

Mesh :

 

Keywords Intsia palembanica, (-)-robidanol, tyrosinase, kinetic inhibition, monophenolase
How to cite this article

:



L.K. Darusman, I. Batubara, T. Mitsunaga, M. Rahminiwati, E. Djauhari and K. Yamauchi, 2012. Tyrosinase Kinetic Inhibition of Active Compounds from Intsia palembanica. Research Journal of Medicinal Plant, 6(8): 615-620. DOI: 10.3923/rjmp.2012.615.620
url: http://scialert.net/abstract/?doi=rjmp.2012.615.620
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