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Glucose Biosensor using Selected Indonesian Bacteria

Dyah Iswantini1*, Novik Nurhidayat2 & Trivadila1



1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Darmaga Campus, Bogor 16680, Indonesia;
2Research Center for Biology, Lembaga Ilmu Pengetahuan Indonesia, Jalan Raya Jakarta-Bogor Km. 46 Cibinong, Bogor 16911, Indonesia
*Email: This email address is being protected from spambots. You need JavaScript enabled to view it.  

Issue Date : February 2011
Publisher : Microbiology Indonesia
Series / Report No :
ISSN 1978-3477, eISSN 2087-8575
Physical Description :
2011 Vol. 5 No. 1, p 9-14
Language : en


Microbial glucose sensors have been developed using Escherichia coli bacterial strains from Japan. However, there is interest in developing local bacteria as glucose sensors in Indonesia. In this research, the stability and the potential of a selected number of Indonesian bacteria as glucose biosensors was explored. Results of this study indicate that three of them, E coliBacillus subtilis, and Thermus filiformis exhibit properties of high viability and stability at high temperature (30-60ºC). Spectrophotometrical and electrochemical measurements showed significant absorbance values and highly stable current features for E coli as indicated by its high capacity to produce glucose dehydrogenase. E. coli, B. subtilis, and T. filiformis produced currents of 3.25 μA, 0.2 μA, and 0.02 μA respectively, and E. coli also produced a much higher activity of glucose dehydrogenase. Electrochemical measurement using E. coli-modified carbon paste electrode allowed the determination of glucose concentration of up to 20 mM. Therefore, Indonesian E. coli has a high stability and can be used as a glucose biosensor.
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Mesh :


glucose biosensor, electrochemistry, bacteria, Escherichia coli, Bacillus subtilis, Thermus filiformis
How to cite this article

Iswantini, D., N, Nurhidayat & Trivadila. 2011. Glucose Biosensor using Selected Indonesian Bacteria. Microbiology Indonesia, 5(1): 9-14.
url: http://biofarmaka.ipb.ac.id/publication/journal/112-glucose-biosensor-using-selected-indonesian-bacteria
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