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| Please use this identifier to cite or link to this item http://biofarmaka.ipb.ac.id/publication/journal/112-glucose-biosensor-using-selected-indonesian-bacteria |
| Title | : |
Glucose Biosensor using Selected Indonesian Bacteria |
| Authors |
: | Dyah Iswantini1*, Novik Nurhidayat2 & Trivadila1 |
| Corporates |
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1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Darmaga Campus, Bogor 16680, Indonesia; |
| Issue Date | : | February 2011 |
| Publisher | : | Microbiology Indonesia |
| Series / Report No | : |
ISSN 1978-3477, eISSN 2087-8575 |
| Physical Description | : |
2011 Vol. 5 No. 1, p 9-14 |
| Language | : | en |
| Abstract |
: |
Microbial glucose sensors have been developed using Escherichia coli bacterial strains from Japan. However, there is interest in developing local bacteria as glucose sensors in Indonesia. In this research, the stability and the potential of a selected number of Indonesian bacteria as glucose biosensors was explored. Results of this study indicate that three of them, E coli, Bacillus subtilis, and Thermus filiformis exhibit properties of high viability and stability at high temperature (30-60ºC). Spectrophotometrical and electrochemical measurements showed significant absorbance values and highly stable current features for E coli as indicated by its high capacity to produce glucose dehydrogenase. E. coli, B. subtilis, and T. filiformis produced currents of 3.25 μA, 0.2 μA, and 0.02 μA respectively, and E. coli also produced a much higher activity of glucose dehydrogenase. Electrochemical measurement using E. coli-modified carbon paste electrode allowed the determination of glucose concentration of up to 20 mM. Therefore, Indonesian E. coli has a high stability and can be used as a glucose biosensor. |
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| Keywords |
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glucose biosensor, electrochemistry, bacteria, Escherichia coli, Bacillus subtilis, Thermus filiformis |
| How to cite this article |
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Iswantini, D., N, Nurhidayat & Trivadila. 2011. Glucose Biosensor using Selected Indonesian Bacteria. Microbiology Indonesia, 5(1): 9-14. url: http://biofarmaka.ipb.ac.id/publication/journal/112-glucose-biosensor-using-selected-indonesian-bacteria |
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