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Synergistic Anticandidal Activity of Xanthorrhizol in Combination with Ketoconazole or Amphotericin B

¹Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea
²Biopharmaca Research Center and Research Center for Biotechnology and Bioresources, Bogor Agricultural University, Bogor, Indonesia;
³Department of Biomaterials and Engineering, Yonsei University, Seoul, Korea; and
⁴Department of Laboratory Medicine, Research Institute of Bacterial Resistance, College of Medicine, Yonsei University, Seoul, Korea

Candida species are responsible for the fourth most common nosocominal bloodstream infection. Xanthorrhizol, a sesquiterpene compound isolated from Curcuma xanthorrhiza Roxb. has been reported to have anticandidal activity. The aim of this study is to investigate the synergistic anticandidal effect of xanthorrhizol in combination with ketoconazole or amphotericin B against Candida albicans, Candida glabrata, Candida guilliermondii, Candida krusei, Candida parapsilosis, and Candida tropicalis. Mostly, xanthorrhizol in combination with ketoconazole or amphotericin B exhibited the synergistic anticandidal effects against all species of Candida tested. In combination with xanthorrhizol, the concentration of ketoconazole or amphotericin B for inhibiting the growth of the tested Candida species could be reduced by Z50%. Time–kill curves showed that 1/2 minimum inhibitory concentration (MIC) dose of xanthorrhizol, amphotericin B, or ketoconazole alone against each of the six Candida species did not inhibit the growth of all Candida species tested. However, 1/2 MIC dose of xanthorrhizol in combination with 1/2 MIC dose of ketoconazole or 1/2 MIC dose of amphotericin B exhibited growth inhibition of all Candida species tested and reduced viable cells by several logs within 4 h. These results support the potential use of xanthorrhizol as an anticandidal agent, and it can be used complementarily with other conventional antifungal agents.

http://biofarmaka.ipb.ac.id/publication/journal/143-synergistic-anticandidal-activity-of-xanthorrhizol-in-combination-with-ketoconazole-or-amphotericin-b

Rukayadi, Y., KY Lee, MS Lee, D. Yong & JK Hwang. 2009. Synergistic Anticandidal Activity of Xanthorrhizol in Combination with Ketoconazole or Amphotericin B. FEMS Yeast Res, 9(8): 1302–1311
url: http://biofarmaka.ipb.ac.id/publication/journal/143-synergistic-anticandidal-activity-of-xanthorrhizol-in-combination-with-ketoconazole-or-amphotericin-b