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Leydig Cells Encapsulation with Alginate-Chitosan: Optimization of Microcapsule Formation

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JOURNAL 2012
Leydig Cells Encapsulation with Alginate-Chitosan: Optimization of Microcapsule Formation

Please use this identifier to cite or link to this item
http://biofarmaka.ipb.ac.id/publication/journal/630-leydig-cells-encapsulation-with-alginate-chitosan-optimization-of-microcapsule-formation
Title
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Leydig Cells Encapsulation with Alginate-Chitosan: Optimization of Microcapsule Formation
Authors

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 		 Irmanida Batubara1,2,*, Devi Rahayu1, Kusdiantoro Mohamad2, Wahono Esthi Prasetyaningtyas2
Corporate






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1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia. Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
2Department of Anatomy Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
3Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No. 3, Bogor 16128, Indonesia.
*Coressponding Author: I. Batubara, Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No. 3, Bogor 16128. Telp +62-251-8373561 Fax +62+251+8347525 
Issue Date : June, 2012
Publisher : Journal of Encapsulation and Adsorption Sciences
Series / Report No :
 		 DOI: 10.4236/jeas.2012.22003
 
Physical Description :
 		 2012, Volume: 2, Issue: 2, Page No: 15-20
 
Language : en
Abstract











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 		 This research aimed to optimize the formation of microcapsules from alginate and chitosan for Leydig cells encapsulation. Alginate was used as the first coating agent while chitosan was the second layer. Various concentrations of alginate and CaCl2 were applied utilizing the extrusion method and the best concentration was determined based on their formation time, shape and diameter of microcapsules. Alginate microcapsule was applied with chitosan in various concentrations. The best chitosan concentration was selected based on its mechanical stability. The results showed that the minimum concentration of alginate was 1.5% (w/v) with viscosity of 33.8 cPs, resulted to spherical microcapsules with diameters of 230 - 270 μm. The optimum concentration of chitosan as the second coating agent was 0.5% (w/v), resulted to spherical microcapsules with mechanical stability of 4 hours. Leydig cells were trapped inside the microcapsule with a density that is proportional to the concentration of cells used in the encapsulation.
Source url
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http://biofarmaka.ipb.ac.id/publication/journal/630-leydig-cells-encapsulation-with-alginate-chitosan-optimization-of-microcapsule-formation
Mesh :

 
Keywords :  cell encapsulation, chitosan; alginate; microcapsule; Leydig cells
How to cite this article

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 I. Batubara, D. Rahayu, K. Mohamad and W. Prasetyaningtyas, Leydig Cells Encapsulation with Alginate-Chitosan: Optimization of Microcapsule Formation, Journal of Encapsulation and Adsorption Sciences, Vol. 2 No. 2, 2012, pp. 15-20. doi: 10.4236/jeas.2012.22003.
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