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Simultaneous Determination of Gingerols and Shogaol using Capillary Liquid Chromatography and Its Application In Discrimination of Three Ginger Varieties from Indonesia

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Thursday, 31 January 2013 09:17
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JOURNAL 2013
Simultaneous Determination of Gingerols and Shogaol using Capillary Liquid Chromatography and Its Application In Discrimination of Three Ginger Varieties from Indonesia

Please use this identifier to cite or link to this item
http://biofarmaka.ipb.ac.id/publication/journal/634-simultaneous-determination-of-gingerols-and-shogaol-using-capillary-liquid-chromatography-and-its-application-in-discrimination-of-three-ginger-varieties-from-indonesia
Title
 
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Simultaneous Determination of Gingerols and Shogaol using Capillary Liquid Chromatography and Its Application In Discrimination of Three Ginger Varieties from Indonesia
Authors
 :
 Mohamad Rafia,b, Lee Wah Lima, Toyohide Takeuchia,*, Latifah Kosim Darusmanb,c
Corporate






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aDepartment of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
bDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University Jalan Agatis Kampus IPB Dramaga, Bogor16680, Indonesia
cBiopharmaca Research Center - Research and Community Empowerment Institute, Bogor Agricultural University, Jalan Taman Kencana No.3 Kampus IPB Taman Kencana, Bogor 16128,Indonesia
*Corresponding Author: Tel/Fax: +81 582932806, e-mail address: This email address is being protected from spambots. You need JavaScript enabled to view it. (T.Takeuchi).
Issue Date : January 15, 2013
Publisher : Talanta
Series / Report No :

 DOI: 10.1016/j.talanta.2012.09.057
 
Physical Description :
 		 2013, Volume: 132, pages: 28–32
 
Language : en
Abstract














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 		 A new method using reversed phase capillary liquid chromatography was developed for simultaneous determination of four bioactive compounds found in ginger (Zingiber officinale) namely, 6-, 8-, 10-gingerol, and 6-shogaol. The separation of these four compounds was performed using C30 as the stationary phase and 60% acetonitrile as the mobile phase in isocratic elution mode with a flow rate of 5 μL/min. All four compounds were separated within 25 min with good resolution. As the evaluation of method validation, a linear regression of the four compounds was obtained within the tested range with correlation coefficients ≥ 0.9995. The limits of detection and quantitation were between 0.034-0.039 μg/mL and 0.112-0.129 μg/mL, respectively. Intra- and inter-day precision expressed as relative standard deviations (RSD) were less than 3.1%, and the accuracy based on recovery test was ranging from 97% to 105%. Stability of the analytes within 1 day was found in the range between 1.34% and 2.93% (RSD). In addition, based on the amount of these four compounds combining with the discriminant analysis, a reliable and accurate method was developed for discrimination of three ginger varieties found in Indonesia. The results indicated that the developed method could be used as quality control for ginger raw material and its related products.

Source url

 

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http://biofarmaka.ipb.ac.id/publication/journal/634-simultaneous-determination-of-gingerols-and-shogaol-using-capillary-liquid-chromatography-and-its-application-in-discrimination-of-three-ginger-varieties-from-indonesia
Mesh :

 
Keywords
 		 :
 		 Zingiber officinale; Ginger; Capillary liquid chromatography; Gingerol; Shogaol; Discriminant analysis
How to cite this article

 :

Mohamad Rafi, Lee Wah Lim, Toyohide Takeuchi, Latifah Kosim Darusman. Simultaneous Determination of Gingerols and Shogaol using Capillary Liquid Chromatography and Its Application In Discrimination of Three Ginger Varieties from Indonesia. Talanta, Vol 103, 2013 pp. 28–32. doi: 10.1016/j.talanta.2012.09.057
url: http://www.sciencedirect.com/science/article/pii/S0039914012008132

 

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