Biopharmaca BiofarmakaBrazilin from Caesalpinia sappan Wood as an Anti Acne Agent


Brazilin from Caesalpinia sappan Wood as an Anti Acne Agent

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Brazilin from Caesalpinia sappan Wood as an Anti Acne Agent

Authors  Irmanida Batubara1,2, Tohru Mitsunaga3 and Hideo Ohashi3



1Departement of Chemistry, Faculty of Mathematics & Natural Sciences, Bogor Agricultural University, Indonesia.
2Biopharmaca Research Center, Institute of Research & Community Services, Bogor Agricultural University, Indonesia.
3Deparment of Applied Biological Science, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

Issue Date : February 2010
Publisher  Journal of Wood Science Publisher: Springer Japan
Series / Report No :
eISSN: 1611-4663, pISSN: 1435-0211
DOI 10.1007/s10086-009-1046-0 
Physical Description :
2010, Vol. 56 No. 1 p 77-81
Language : en



In screening experiments for antiacne activity, methanolic and 50% ethanolic extracts of Caesalpinia sappan wood showed the most potent activity out of 28 species of plants extracts. These extracts showed inhibition of Propionibacterium acnes growth, lipase inhibitory activity, and antioxidant activity. In order to isolate the active compound from C. sappan, separation of the extract components was performed by column chromatography and preparative high-performance liquid chromatography (HPLC). Brazilin, protosappanin A, and sappanone B were isolated from methanolic extracts. Brazilin showed better antibacterial activity [minimum inhibitory concentration (MIC) = minimum bactericidal concentration (MBC) = 0.50 mg/ml] than protosappanin A (MIC = MBC = 1.00 mg/ml) and sappanone B (MIC = MBC > 2.00 mg/ml). The 50% inhibitory concentration (IC50) for lipase inhibition was lowest for brazilin (6 μM), which showed strong inhibition compared with protosappanin A (100 μM) and chloramphenicol (677 μM, positive control). The antioxidant activity of brazilin (IC50 8.8 μM) was not significantly different from protosappanin A (9.1 μM) and (+)-catechin (10.2 μM). The antioxidant activity of brazilin and protosappanin A were higher than sappanone B (IC50 14.5 μM). Brazilin is considered to have suffi ciently potent activity for use as an antiacne agent.

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Mesh :


Keywords  Caesalpinia sappan, Brazilin, Protosappanin A, Propionibacterium acnes, Lipase inhibitor
How to cite this article  :


Batubara, I., T. Mitsunaga and H. Ohashi. 2010. Brazilin from Caesalpinia sappan Wood as an Anti Acne Agent. Journal of Wood Science, 56(1): 77-81.



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